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anti shp2  (Proteintech)


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    Structured Review

    Proteintech anti shp2
    Anti Shp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+shp2/SHP2+Antibody/pmc13015236-85-25-26
    Average 93 stars, based on 34 article reviews
    anti shp2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells.
    Article Snippet: Brief Communication Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells

    Article Title: Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.
    Article Snippet: Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations account for 35% of the genetic alterations in nonsmall cell lung cancer (NSCLC).. The Src-homology region 2-containing protein tyrosine phosphatase 2 (SHP2), encoded by PTPN11, is closely involved in RAS downstream pathways and development of many tumors by affecting cell proliferation, differentiation, and immunity.. Targeting SHP2 with small molecules may be a promising avenue for the treatment of KRAS-mutant (mut) NSCLC.

    Western Blot:

    Article Title: A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity
    Article Snippet: The protein concentrations were 185 measured utilizing the enhanced BCA protein assay kit (Beyotime). .. Following this, the cell lysates 186 Jo urn al Pr e-p roo f were heated for 5 min and then underwent separation prior to Western blot analysis, where the 187 primary antibodies utilized included anti-SHP2 (Proteintech), anti-phosphorylated-SHP2 188 (Invitrogen), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Proteintech), anti-ERK 189 (CST, Danvers, MA, USA), anti-phosphorylated-ERK (CST), anti-AKT (Proteintech), anti-190 phosphorylated-AKT (Proteintech), anti-ZAP70 (Proteintech), anti-phosphorylated-ZAP70 (R&D, 191 Minneapolis, MN, USA), anti-SYK (R&D, Minneapolis, MN, USA), anti-phosphorylated-SYK 192 (R&D), anti-PD-1 (Proteintech), anti-SIRPα (Abcam, Cambridge, UK), and anti-NKG2A (Abcam). .. 193 For secondary antibodies, HRP-conjugated anti-rabbit and anti-mouse antibodies (Proteintech) were 194 employed.

    Article Title: A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity
    Article Snippet: The protein concentrations were measured utilizing the enhanced BCA protein assay kit (Beyotime, Shanghai, China). .. Following this, the cell lysates were heated for 5 min and then underwent separation prior to Western blot analysis, where the primary antibodies utilized included anti-SHP2 (Proteintech, Rosemont, IL, USA), anti-phosphorylated-SHP2 (Invitrogen, Carlsbad, CA, USA), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Proteintech, Rosemont, IL, USA), anti-ERK (CST, Danvers, MA, USA), anti-phosphorylated-ERK (CST, Danvers, MA, USA), anti-AKT (Proteintech, Rosemont, IL, USA), anti-phosphorylated-AKT (Proteintech, Rosemont, IL, USA), anti-ZAP70 (Proteintech, Rosemont, IL, USA), anti-phosphorylated-ZAP70 (R&D, Minneapolis, MN, USA), anti-SYK (R&D, Minneapolis, MN, USA), anti-phosphorylated-SYK (R&D, Minneapolis, MN, USA), anti-PD-1 (Proteintech, Rosemont, IL, USA), anti-SIRPα (Abcam, Cambridge, UK), and anti-NKG2A (Abcam, Cambridge, UK). .. For secondary antibodies, HRP-conjugated anti-rabbit and anti-mouse antibodies (Proteintech, Rosemont, IL, USA) were employed.

    Incubation:

    Article Title: The role of tyrosine phosphatase Shp2 in spermatogonial differentiation and spermatocyte meiosis
    Article Snippet: .. In brief, separated proteins on nylon membranes were incubated with specific primary antibodies such as anti-Shp2 (1:1000) and anti-tubulin (1:1000, Proteintech, Wuhan, China) at 4°C overnight and then with secondary antibodies (ZSbio) at 37°C for 2 h. The results were visualized with enhanced chemiluminescence. .. Grayscale bands were quantified using Quantity One software (Bio-Rad, Hercules, CA, USA).

    Expressing:

    Article Title: Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model
    Article Snippet: .. The relative expression levels of the target genes were normalized to the reference gene β-actin using the 2–ΔΔCt method (Gilyazova et al., 2023). anti-SHP2 (1:200, Proteintech, Cat# 20145-1-AP, RRID: AB_10699877), mouse anti-PKM2 (1:400, Proteintech, Cat# 60268-1-Ig, RRID: AB_2881388), rabbit anti-CD163 (1:200, Proteintech, Cat# 16646-1-AP, RRID: AB_2756528), rabbit antiCD86 (1:200, Affinity, Cat# DF6332, RRID: AB_2838296), and rabbit anti-c-caspase3 (1:250, Abcam, Cambridge, UK, Cat# ab32042, RRID: AB_725947). .. The following day, the sections or coverslips were incubated with the following secondary antibodies for 30 to 60 minutes at room temperature: CoraLite488-conjugated goat anti-mouse IgG+L (1:400, Proteintech, Cat# SA00013-1, RRID: AB_2810983) and CoraLite594-conjugated goat anti-rabbit IgG (H+L) (1:400, Proteintech, Cat# SA00013-4, RRID: AB_2810984).



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    NFIC promotes OGN and PTEN expression while inhibiting NF-κB, <t>SHP2,</t> and phosphorylated SHP2 expression. A: Western blot analysis of NFIC, OGN, p-NF-κB, SHP2, and p-SHP2 protein bands in six groups (NFIC-NC, NFIC-OE, NF-κB NC, NF-κB NC, PHPS1 NC, PHPS1 OE), with statistical analysis of relative protein expression levels. B: Western blot analysis of PTEN and HEY1 protein bands in six groups, along with statistical analysis of relative protein expression levels. Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, * P < 0.05; ** P < 0.01; nsP > 0.05.
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    NFIC promotes OGN and PTEN expression while inhibiting NF-κB, <t>SHP2,</t> and phosphorylated SHP2 expression. A: Western blot analysis of NFIC, OGN, p-NF-κB, SHP2, and p-SHP2 protein bands in six groups (NFIC-NC, NFIC-OE, NF-κB NC, NF-κB NC, PHPS1 NC, PHPS1 OE), with statistical analysis of relative protein expression levels. B: Western blot analysis of PTEN and HEY1 protein bands in six groups, along with statistical analysis of relative protein expression levels. Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, * P < 0.05; ** P < 0.01; nsP > 0.05.
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    NFIC promotes OGN and PTEN expression while inhibiting NF-κB, <t>SHP2,</t> and phosphorylated SHP2 expression. A: Western blot analysis of NFIC, OGN, p-NF-κB, SHP2, and p-SHP2 protein bands in six groups (NFIC-NC, NFIC-OE, NF-κB NC, NF-κB NC, PHPS1 NC, PHPS1 OE), with statistical analysis of relative protein expression levels. B: Western blot analysis of PTEN and HEY1 protein bands in six groups, along with statistical analysis of relative protein expression levels. Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, * P < 0.05; ** P < 0.01; nsP > 0.05.
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    Image Search Results


    NFIC promotes OGN and PTEN expression while inhibiting NF-κB, SHP2, and phosphorylated SHP2 expression. A: Western blot analysis of NFIC, OGN, p-NF-κB, SHP2, and p-SHP2 protein bands in six groups (NFIC-NC, NFIC-OE, NF-κB NC, NF-κB NC, PHPS1 NC, PHPS1 OE), with statistical analysis of relative protein expression levels. B: Western blot analysis of PTEN and HEY1 protein bands in six groups, along with statistical analysis of relative protein expression levels. Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, * P < 0.05; ** P < 0.01; nsP > 0.05.

    Journal: PLOS One

    Article Title: NFIC suppressed the development of Glioma via modulating the balance of SHP2/PI3K and NF-κB/PTEN Signaling

    doi: 10.1371/journal.pone.0341816

    Figure Lengend Snippet: NFIC promotes OGN and PTEN expression while inhibiting NF-κB, SHP2, and phosphorylated SHP2 expression. A: Western blot analysis of NFIC, OGN, p-NF-κB, SHP2, and p-SHP2 protein bands in six groups (NFIC-NC, NFIC-OE, NF-κB NC, NF-κB NC, PHPS1 NC, PHPS1 OE), with statistical analysis of relative protein expression levels. B: Western blot analysis of PTEN and HEY1 protein bands in six groups, along with statistical analysis of relative protein expression levels. Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, * P < 0.05; ** P < 0.01; nsP > 0.05.

    Article Snippet: The membranes were blocked in TBST buffer containing 5% skimmed milk at 37°C for 2 hours, followed by overnight incubation at 4°C with the following primary antibodies: NFIC (CST, #11911, 1:1000), OGN (CST, #24083, 1:1000), NF-κB (CST, #8242, 1:1000), SHP2 (CST, #3752, 1:1000), p-SHP2 (CST, #13379, 1:1000), PI3K (CST, #4292, 1:1000), AKT (CST, #4060, 1:2000), Cyclin A1 (CST, #4656, 1:2000), Cyclin D1 (CST, #2922, 1:1000), MMP-3 (CST, #14351, 1:1000), and MMP-9 (CST, #3852, 1:1000).

    Techniques: Expressing, Western Blot, Standard Deviation

    NFIC promotes OGN and PTEN expression while suppressing NF-κB, SHP2, and phosphorylated SHP2 expression. A: Western blot detection of p-PIK, p-AKT, p-STAT3, GAPDH protein bands, and statistical analysis of relative protein expression levels; B: Western blot detection of Cyclin A1, Cyclin D1, MMP-3, and MMP-9 protein bands in the six groups, and statistical analysis of relative protein expression levels. Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, * P < 0.05; ** P < 0.01; nsP > 0.05.

    Journal: PLOS One

    Article Title: NFIC suppressed the development of Glioma via modulating the balance of SHP2/PI3K and NF-κB/PTEN Signaling

    doi: 10.1371/journal.pone.0341816

    Figure Lengend Snippet: NFIC promotes OGN and PTEN expression while suppressing NF-κB, SHP2, and phosphorylated SHP2 expression. A: Western blot detection of p-PIK, p-AKT, p-STAT3, GAPDH protein bands, and statistical analysis of relative protein expression levels; B: Western blot detection of Cyclin A1, Cyclin D1, MMP-3, and MMP-9 protein bands in the six groups, and statistical analysis of relative protein expression levels. Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, * P < 0.05; ** P < 0.01; nsP > 0.05.

    Article Snippet: The membranes were blocked in TBST buffer containing 5% skimmed milk at 37°C for 2 hours, followed by overnight incubation at 4°C with the following primary antibodies: NFIC (CST, #11911, 1:1000), OGN (CST, #24083, 1:1000), NF-κB (CST, #8242, 1:1000), SHP2 (CST, #3752, 1:1000), p-SHP2 (CST, #13379, 1:1000), PI3K (CST, #4292, 1:1000), AKT (CST, #4060, 1:2000), Cyclin A1 (CST, #4656, 1:2000), Cyclin D1 (CST, #2922, 1:1000), MMP-3 (CST, #14351, 1:1000), and MMP-9 (CST, #3852, 1:1000).

    Techniques: Expressing, Western Blot, Standard Deviation

    NFIC inhibits glioblastoma cell proliferation and invasion, while NF-κB promotes these processes. A: Co-immunoprecipitation (CO-IP) analysis of NFIC and PTEN protein bands in six sample groups; B: CO-IP analysis of OGN and NF-κB protein bands in six sample groups; C: CO-IP analysis of NF-κB and SHP2 protein bands in six sample groups; D: CO-IP analysis of NFIC and OGN protein bands in six sample groups; Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference; * P < 0.05; ** P < 0.01; nsP > 0.05.

    Journal: PLOS One

    Article Title: NFIC suppressed the development of Glioma via modulating the balance of SHP2/PI3K and NF-κB/PTEN Signaling

    doi: 10.1371/journal.pone.0341816

    Figure Lengend Snippet: NFIC inhibits glioblastoma cell proliferation and invasion, while NF-κB promotes these processes. A: Co-immunoprecipitation (CO-IP) analysis of NFIC and PTEN protein bands in six sample groups; B: CO-IP analysis of OGN and NF-κB protein bands in six sample groups; C: CO-IP analysis of NF-κB and SHP2 protein bands in six sample groups; D: CO-IP analysis of NFIC and OGN protein bands in six sample groups; Data are expressed as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference; * P < 0.05; ** P < 0.01; nsP > 0.05.

    Article Snippet: The membranes were blocked in TBST buffer containing 5% skimmed milk at 37°C for 2 hours, followed by overnight incubation at 4°C with the following primary antibodies: NFIC (CST, #11911, 1:1000), OGN (CST, #24083, 1:1000), NF-κB (CST, #8242, 1:1000), SHP2 (CST, #3752, 1:1000), p-SHP2 (CST, #13379, 1:1000), PI3K (CST, #4292, 1:1000), AKT (CST, #4060, 1:2000), Cyclin A1 (CST, #4656, 1:2000), Cyclin D1 (CST, #2922, 1:1000), MMP-3 (CST, #14351, 1:1000), and MMP-9 (CST, #3852, 1:1000).

    Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay, Standard Deviation

    NFIC binds to the promoter regions of OGN and PTEN and regulates their transcription, leading to increased expression of these two genes. Immunohistochemical staining results for NFIC, PTEN, OGN, NF-κB, and p-SHP2, along with statistical analysis of staining. Data are presented as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, *P < 0.05, **P < 0.01.

    Journal: PLOS One

    Article Title: NFIC suppressed the development of Glioma via modulating the balance of SHP2/PI3K and NF-κB/PTEN Signaling

    doi: 10.1371/journal.pone.0341816

    Figure Lengend Snippet: NFIC binds to the promoter regions of OGN and PTEN and regulates their transcription, leading to increased expression of these two genes. Immunohistochemical staining results for NFIC, PTEN, OGN, NF-κB, and p-SHP2, along with statistical analysis of staining. Data are presented as mean ± standard deviation. N = 3, P < 0.05 indicates statistically significant difference, *P < 0.05, **P < 0.01.

    Article Snippet: The membranes were blocked in TBST buffer containing 5% skimmed milk at 37°C for 2 hours, followed by overnight incubation at 4°C with the following primary antibodies: NFIC (CST, #11911, 1:1000), OGN (CST, #24083, 1:1000), NF-κB (CST, #8242, 1:1000), SHP2 (CST, #3752, 1:1000), p-SHP2 (CST, #13379, 1:1000), PI3K (CST, #4292, 1:1000), AKT (CST, #4060, 1:2000), Cyclin A1 (CST, #4656, 1:2000), Cyclin D1 (CST, #2922, 1:1000), MMP-3 (CST, #14351, 1:1000), and MMP-9 (CST, #3852, 1:1000).

    Techniques: Expressing, Immunohistochemical staining, Staining, Standard Deviation

    NFIC promotes OGN and PTEN expression while suppressing NF-κB, SHP2, p-SHP2, PI3K, AKT, Cyclin A1, Cyclin D1, MMP-3, and MMP-9 expression. NF-κB promotes SHP2 expression, while OGN and PTEN inhibit p-SHP2 expression. NFIC suppresses glioma cell proliferation and invasion, whereas NF-κB promotes these processes.

    Journal: PLOS One

    Article Title: NFIC suppressed the development of Glioma via modulating the balance of SHP2/PI3K and NF-κB/PTEN Signaling

    doi: 10.1371/journal.pone.0341816

    Figure Lengend Snippet: NFIC promotes OGN and PTEN expression while suppressing NF-κB, SHP2, p-SHP2, PI3K, AKT, Cyclin A1, Cyclin D1, MMP-3, and MMP-9 expression. NF-κB promotes SHP2 expression, while OGN and PTEN inhibit p-SHP2 expression. NFIC suppresses glioma cell proliferation and invasion, whereas NF-κB promotes these processes.

    Article Snippet: The membranes were blocked in TBST buffer containing 5% skimmed milk at 37°C for 2 hours, followed by overnight incubation at 4°C with the following primary antibodies: NFIC (CST, #11911, 1:1000), OGN (CST, #24083, 1:1000), NF-κB (CST, #8242, 1:1000), SHP2 (CST, #3752, 1:1000), p-SHP2 (CST, #13379, 1:1000), PI3K (CST, #4292, 1:1000), AKT (CST, #4060, 1:2000), Cyclin A1 (CST, #4656, 1:2000), Cyclin D1 (CST, #2922, 1:1000), MMP-3 (CST, #14351, 1:1000), and MMP-9 (CST, #3852, 1:1000).

    Techniques: Expressing